772 search results for “protein” in the Public website
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Knowledge of DNA repair in the fight against tumour cells
What is the most effective way to eliminate tumour cells? The DNA repair mechanism could play an important role in increasing the effectiveness of chemotherapy in the fight against cancerous cells. If we are to influence this mechanism, we need fundamental knowledge about how the mechanism works.
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Freedom of additional signals on genes: on the combination of DNA mechanics, genetics and translation speed
DNA carries various forms of information. Out of these forms of information the most well-known is classical genetic information.
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Human skin equivalents for atopic dermatitis: investigating the role of filaggrin in the skin barrier
Promotor: Prof.dr. J.A. Bouwstra, Co-promotor: Dr. A. El Ghalbzouri
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Impaired barrier of inflammatory skin diseases
Focus in skin research
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Optoplasmonic detection of single particles and molecules in motion
Detecting nanoscopic objects plays an important role in nanoscience in particular, in the rapidly growing field of nanobiology. The forebear to modern super-resolution microscopy for single molecule investigation, is fluorescence microscopy.
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Software
Parassign is a program to assign protein nuclei solely on the basis of pseudocontact shifts (PCS). The PCS from several paramagnetic tags are required, in order to produce several restraints per nucleus.
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Thiosugars: reactivity, methodology and applications
Carbohydrates, alongside proteins and nucleic acids, constitute a crucial and versatile family of biomolecules present in all life forms. They manifest as monosaccharides, oligosaccharides, and polysaccharides, covalently bonded to proteins and fats. Carbohydrates are integral to plant and arthropod…
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Histone-DNA assemblies in archaea. Shaping the genome on the edge of life
All life on earth contains DNA, which is used to store biological information. Organisms compact their DNA in order for it to fit inside their cell(s).
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Bacterial Chromatin
The relative simplicity of the bacterial cell, short generation times and well defined and inexpensive culturing conditions have significantly contributed to our understanding of many complex biological systems. Yet the workings of the bacterial genome, seemingly impossibly compressed within a tiny…
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Fluorescence Correlation Spectroscopy on Electron Transfer Reaction: Probing Inter- and Intramolecular Redox Processes
Promotores: G.W. Canters, T.J. Aartsma
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Functional analysis of Dof transcription factors controlling heading date and PPDK gene expression in rice
Promotor: P.J.J. Hooykaas, Co-Promotores: P.B.F. Ouwerkerk, M. Wang
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A new method to reconstruct the structure from crystal images
Promotor: J.P. Abrahams, Co-promotor: T. Grüne
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Regulation of autophagy-related mechanisms during bacterial infection
Autophagy is a fundamental degradative process, maintaining cellular homeostasis and functions in host defense against intracellular pathogens, including mycobacteria and Salmonella.
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Molecular characterization of copper-dependent enzymes involved in Streptomyces morphology
Promotor: G.P. van Wezel, Co-promotor: D. Claessen
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Gene regulation in embryonic development
The human body consists of hundreds, perhaps thousands of different types of cells, each with different morphologies and functions, despite having the same genome.
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Expertise
The CMCB brings together a diverse range of unique expertise in microbial cell biology. Members of the CMCB investigate both model and non-model organisms, bacteria and archaea, pathogens and non-pathogens.
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Identification and characterization of developmental genes in streptomyces
Promotor: Prof.dr. G.P. van Wezel
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More JAZ in the orchestration of jasmonate-mediated plant defense
Promotor: Prof.dr. J. Memelink
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Collaborative and effective drug development
There are many complex links in the chain that provides patients with new drugs: from fundamental science, to clinical tests, to production. The entire chain can be found in Leiden. Leiden University, the Leiden University Medical Center (LUMC) and the businesses at the Leiden Bio Science Park (LBSP)…
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Biologics Formulation
A major challenge in the formulation of biopharmaceuticals is to overcome their inherent instability during production, storage, transportation, handling and administration. We are studying underlying mechanisms of degradation of biopharmaceuticals (including therapeutic proteins, vaccines and advanced…
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Solid State NMR and modelling of photoinduced energy and electron transfer
Huub de Groot is professor in Biophysical Organic Chemistry. With his team he works in the field of photosynthesis and artificial photosynthesis. The molecular basis for photosynthesis is formed by protein complexes and organelles that contain chlorophyll molecules. The antenna systems herein capture…
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Resetting the immune system to cure diabetes and rheumatism
In autoimmune diseases such as rheumatism and diabetes the immune system attacks autologous proteins. Leiden researchers are trying to discover how this comes about.
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Chemical tools to study the cannabinoid receptor type 2
The cannabinoid receptor type 2 (CB2R) is associated with several inflammatory diseases with an unmet medical need (e.g. Alzheimers, multiple sclerosis, reumatoid arthritis). Development of new chemical biology strategies to study this protein is essential to aid future development of drugs for these…
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Spherical mosaics from different tiles
What are 'defects' and mechanical properties of mosaics of different shapes of tiles?
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Non-covalent Interactions
Many biological processes occur by biomolecules coming together and organizing into specific clusters without being bound to each other. These interactions range from clustering, transiently interacting (kiss and run), or assembling to multimeric units that can only function in conjunction. The hydrogen…
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CD40-targeted dendritic cell delivery of PLGA-nanoparticle vaccines induce potent anti-tumor responses
Dendritic cells (DC) play a prominent role in the priming of CD8(+) T cells. Vaccination is a promising treatment to boost tumor-specific CD8(+) T cells which is crucially dependent on adequate delivery of the vaccine to DC.
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Unspinning chromatin: molecular mechanisms of chromatin remodeling
How do you fit two meters of DNA in a tiny compartment and at the same time are able to access the right parts of it at the right times?
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Development of cross-protective influenza A vaccines based on cellular responses
Seasonal influenza vaccines provide protection against matching influenza A virus (IAV) strains mainly through the induction of neutralizing serum IgG antibodies. However, these antibodies fail to confer a protective effect against mismatched IAV. This lack of efficacy against heterologous influenza…
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Research
Research at the BPOC/SSNMR group is comprised of the following research themes:
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Mass Spectrometry-Based Degradomics Analysis of Toxoid Vaccines
The chemical and structural heterogeneity of toxoid vaccines makes their analysis challenging. However, detailed insights on a molecular level can be obtained by mass spectrometry.
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An ex vivo human skin model for studying skin barrier repair
In the studies described in this study, we introduce a novel ex vivo human skin barrier repair model. To develop this, we removed the upper layer of the skin, the stratum corneum (SC) by a reproducible cyanoacrylate stripping technique.
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NeCEN
With the powerful electron microscopes at NeCEN we study abnormalities in cells of blood vessels in cardiovascular disease, the interaction between pathogens and hosts in infectious diseases, molecular processes that cause cancer and the interaction between drugs and target proteins. Drug development…
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Overview
Our research is aimed at a smoother and speedier transition from preclinical research to pharmacological interventions, and the delivery of drugs in the human body by use of small molecules and biologics.
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Arabidopsis AGC3 kinases and PIN plasma membrane abundance
The plant hormone auxin plays a central role in the growth and development of plants. Auxin acts in a concentration dependent manner and polar cell-to-cell transport of this hormone determines its distribution in the tissues of plants. This polar auxin transport is mediated by several families of auxin…
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Evolution and development of orchid flowers and fruits
To gain more insight into the evolutionary development of orchid flowers and fruits, the orchid species Erycina pusilla was studied. The evolutionary origin of the median petaloid sepal, the callus on the labellum, and the stelidia was studied.
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Drug Discovery & Safety
In the research programme Drug Discovery & Safety, we are interested in the efficacy and safety of new drugs and novel means to decipher these aspects. Hence, we employ advanced imaging and high-throughput screening techniques next to computational approaches such as chem- and bioinformatics.
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Plant Latex: A Potential Resource of Bioactive Chemicals
How and why latex bearing plants select specific chemical compounds for eco-chemical interactions with its biotic factors?
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Genetic and environmental factors determining heterogeneity in preservation stress resistance of Aspergillus niger conidia
Fungal food spoilage often starts with a contamination with spores. Experimental data strongly indicate the existence of subpopulations of spores with different levels of resistance to preservation methods.
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Development and application of cryo-EM tools to study the ultrastructure of microbes in changing environments
Cryogenic electron microscopy (cryo-EM) is a powerful technique used to visualize the inside of cells and to study specific protein complexes. Within this thesis, I describe the use of various cryo-EM techniques to gain insight into the structural changes of the human pathogen, Vibrio cholerae, as it…
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Explant cultures of atopic dermatitis biopsies maintain their epidermal characteristics in vitro
Atopic dermatitis (AD) is a common inflammatory skin disorder characterised by various epidermal alterations. Filaggrin (FLG) mutations are a major predisposing factor for AD and much research has been focused on the FLG protein.
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Chemical biology of sphingolipids: fundamental studies and clinical applications
Overkleeft
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Targeting Human Proteasomes: Substrates, Inhibitors and Prodrugs
Large parts of the research described in this Thesis aims at the development of oligopeptide-masked toxins and their in situ immunoproteasome-mediated activation.
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Heme biosynthesis and regulation in the filamentous fungus Aspergillus niger
Promotors: Prof.dr. P.J. Punt, Prof.dr. C.A.M.J.J. van den Hondel
- Publication highlights
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More effective blocking of CCR2 receptor
The discovery of new medicines is a tedious and lengthy process. On average, over 10,000 molecules need to be studied for one to become a drug and reach the patient. Part of that process are the very costly clinical trials in humans, and candidate drugs often fail due to side effects or lack of efficacy.…
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C.J. Kok Jury Award for Thesis of the Year
Isotopes on exoplanets, a more efficient memory for data centres or new molecules that work against the Zika virus and HIV. Which PhD candidate has written the most impressive dissertation of 2023? The jury of the C.J. Kok Jury Award faces the challenging task of deciding that. Meet the nominees of…
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LED3 Drug Discovery Case Studies
To get a feeling of how we operate at LED3 when it comes to Early Drug Discovery, please browse through our case studies. When you select a case study you’ll find relevant contacts.
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Chemical Biology
Chemical biology research at the Leiden Institute of Chemistry is aimed at understanding biological processes at the molecular level to strengthen the knowledge base of human health and disease. The approach to achieve this goal is a fundamental chemical one; with the aid of chemical probes biological…
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Applications for activity-based probes in biomedical research on glycosidases
Lysosomal glycosidases are acid hydrolases that fragment glycoconjugates in lysosomes.
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Infrastructure
An overview of our infrastructure at the SPP division: