The research described in this thesis focused on the use of bioorthogonal antigens to investigate immunological processes in antigen presenting cells. Bioorthogonal antigens are antigenic proteins produced through recombinant expression in a methionine auxotrophic E. coli strain.

Science

Our immune system protects us against disease, but every now and then, something goes wrong: an enemy invades our bodies or our immune system attacks our own cells and we become ill. Doctors and researchers at the Leiden University Medical Center (LUMC) want to be able to manipulate the immune system…

Mouse models are commonly used to assess the immunogenicity of therapeutic proteins and to investigate the immunological processes leading to antidrug antibodies. The aim of this work was to develop a transgenic (TG) Balb/c mouse model for evaluating the immunogenicity of recombinant human insulin (insulin)…

Antibiotic resistance, caused by widespread use of antibiotics, leads to bacterial infections that are difficult, if not impossible, to treat and is a major worldwide health concern.

We perform multidisciplinary research at molecular, cellular, and organismal levels of animal biology to increase fundamental understanding of health and disease.

In order to be able to develop effective medicine and treatments to prevent or cure autoimmune diseases or cancer we need to understand the mechanisms how they arise and what drives their course.Unravelling the fundamental molecular mechanisms influencing the onset and course of diseases such as allergies,…

This thesis focuses on using liposomes in two different treatment strategies; vaccination (or immunotherapy) and delivery of a small molecule, and in two different disease models; cancer and atherosclerosis.

A vaccine based on sugar coats does have the potential to combat a multi-resistant staphylococcus. That is what Jeroen Codée and his colleagues from Utrecht state in Nature. In doing so, they are contradicting the earlier conclusions of German colleagues.

The quality of vaccines is still often tested by using laboratory animals. That has to change, according to researchers from Leiden University and the vaccinological institute Intravacc. Together they went searching for a test method that makes animal testing unnecessary. The first promising results…

Mashaghi lab conducts research at the interface of physics, engineering and biomedicine. We develop and use theoretical and experimental approaches to address basic and applied research problems.

Can chemical safety assessment be improved with zebrafish embryo-based reporter assays?

Worldwide resurgence of pertussis necessitates the need for improvement of pertussis vaccines and vaccination strategies.

Human and animal cells are very complex: very different chemical processes are going on at the same time, but they are separated from each other because the cells are divided in compartments. These compartments may also have a profound effect on the potential efficacy of therapeutics, because the drug…

How are carbohydrates processed by the immune system? Can carbohydrates be used to trigger T-cells against other conjugated antigens? Can they be used as adjuvants?

This research area is focused on the development and application of novel concepts and models in the emerging area of quantitative systems pharmacology (QSP).

In 2021 many of our students and staff won fantastic prizes and were awarded important research grants. This is our traditional review of these successes as the end of one year marks the beginning of another.

Treatment with special stem cells seems to be a good option for suppressing the immune system in organ transplants. Researchers are trying to learn from processes that take place in the human body during pregnancy.

Tuberculosis causes 1.5 million deaths yearly and anti-tuberculosis therapies are threatened by emergence of drug resistance. Development of innovative drug combinations should be accelerated with the use of translational pharmacological models. Moreover, host-directed therapies (HDT), which stimulate…

Key publications of the Cardiovascular Pharmacology group

Can we bring novel treatment options to the clinic for UM patients with liver metastases?

We perform multidisciplinary research at molecular, cellular, and organismal levels of animal biology to increase fundamental understanding of health and disease.

Atherosclerosis is a chronic inflammatory disease, and vaccination may be an attractive method to induce long lasting protection against endogenous factors that contribute to the development of atherosclerosis. We have shown that for example vaccination against modified LDL or interleukin-12 limits…

Chemical biology research at the Leiden Institute of Chemistry is aimed at understanding biological processes at the molecular level to strengthen the knowledge base of human health and disease. The approach to achieve this goal is a fundamental chemical one; with the aid of chemical probes biological…

Sjaak Neefjes, professor of Chemical Immunology at the LUMC, has been awarded a Spinoza prize of 2.5 million euros. His colleague Ton Schumacher, professor of Immunotechnology affiliated with Leiden University, the LUMC and the Antoni van Leeuwenhoek cancer centre, has been awarded the Stevin prize,…

In 2022, many of our staff and students won fantastic prizes and were awarded important research grants.

Leiden University regularly confers honorary doctorates, and presents awards and prizes.

Sjaak Neefjes, Professor of Chemical Immunology at Leiden University, will receive the NWO Spinoza Prize this year. The Spinoza Committee called Neefjes an ‘exceptional researcher whose intelligence is matched by his boldness’. He will use the 2.5 million euro prize to pursue his research into cancer…

Four Leiden University professors have been appointed to The Royal Holland Society of Sciences and Humanities. Andrea Evers, Erwin Muller, Sjaak Neefjes and Menno Schilthuizen will be joining the oldest ‘learned society’ in the Netherlands.

The Royal Netherlands Academy of Arts and Sciences (KNAW) selects prominent researchers as members based on their scientific achievements. On September 17th, the KNAW will install 21 new members, including Leiden physicist Michel Orrit and chemist Hermen Overkleeft.

Researchers Indu Khatri, Frank Staal and Jacques van Dongen from Leiden University Medical Center (LUMC) have published a perspective article in Frontiers in Immunology about coronavirus and its characteristic ‘spike protein.’ They predict which antibodies would provide the best protection against the…

Cancer is still one of the most common diseases in the world. What exactly is cancer and will we one day be able to cure everyone? In a video from the Universiteit van Nederland Noel de Miranda (Tumour Immunology, LUMC) tells you about his research on new treatments.

KNAW has elected eighteen new members, including two professors at Leiden University and one who studied and obtained his PhD in Leiden. The New Academy members will be installed at a later date this year.

The infectious diseases malaria and tuberculosis are responsible for 2.1 million deaths every year. Leiden researchers are currently testing a new tuberculosis vaccine.

Vaccines are an extraordinary category of biopharmaceuticals. They are usually prophylactic and come in many types, from whole bacteria to peptides and nucleic acids. They are very cost effective and almost every human on the planet is vaccinated. The latter mandates that vaccines have to be extremely…

The goal of hit and lead optimization is to optimize suitable chemical starting points that can modulate a drug target. The methods and technologies used are similar to those in Hit Discovery, but once the compound has shown activity in an animal model, it moves from 'hit' to 'lead.'

LED3 is the combined effort of three excellent institutes of Leiden University. Through joined hands, we are able to give rise to a more effective early drug discovery pipeline. Let us briefly introduce the three institutes.

In this research group, led by Dr. Joost Beltman, the aim is to employ mathematical and computational dynamical modelling approaches in order to quantitatively and mechanistically understand the dynamical behaviour and regulation of intracellular networks of genes, proteins and metabolites as well as…

Physics and Engineering Approaches to Biomedicine and Pharmacology

Two excellent young chemists, Jeroen Codée and Sander van Kasteren, have received an ECHO grant for €260,000 from the Netherlands Organisation for Scientific Research (NWO). This funding will allow them to begin their research projects.

A group of chemists, that includes Sander van Kasteren and Hermen Overkleeft, has discovered that azides, a certain type of chemical compound, can determine whether or not T cells respond to or ignore a vaccine. Their discovery is set to be published in Angewandte Chemie.

Four of Leiden's top researchers have been awarded substantial grants by the European Research Council.

On the 19th of May 2020, our dear colleague and friend Professor Dr. Huib Ovaa has passed away from prostate cancer. Last summer Huib became ill, and in first instance it appeared that he had attracted a relatively harmless infectious disease. Unfortunately, it became soon apparent that he suffered…

In 2020, many of our staff and students have again won prestigious prizes and been awarded important research subsidies.

Kidney transplantation used to be an experimental risky procedure, but is now routinely performed by many countries worldwide.

Prof. Hermen Overkleeft has been awarded the 2015 Jeremy Knowles Award of the Royal Society of Chemistry for his innovative and insightful development of activity-based protein probes for the imaging and identification of enzymes in health and disease.

With cancer, a person’s body cells grow uncontrollably. Putting together a detailed picture of how this comes about makes it possible to develop efficient therapies. Researchers at the Leiden University Medical Centre (LUMC) and Leiden University are working together to gain a better understanding…

In 2023, many of our students and staff won great prizes and secured important research grants.

The application procedure is divided into four parts.