Mechanobiology is a major area in biology, however, mechanics is currently a missing element in drug development pipelines. This has led to a high cost for the society. Mechanotoxicity of drugs often appears as a long-term side effect of drugs, and has led to removal of drugs from the market. Furthermore,…

The aim of this thesis is to study cell-cell interactions and the development of an assay to explore and quantify the exchange of membrane compounds.

The way organisms develop from the initial single-cellular state to a complex final assembly like the human body, and how the final body is maintained throughout life, is one of the greatest mysteries and it’s understanding one of the biggest scientific challenges.

Despite being the object of intense study, embryonic development has been difficult to model due to a number of reasons. First, complex tissues can be comprised of many cell types, of which we probably only know a subset.

The single cell group (SCG) is part of the Metabolomics & Analytics Centre Our research is centered around developing innovating analytical methods tailored for small-volume, or single cell analysis to enable metabolomics-driven systems biology in personalized health strategies. We are always on…

In this thesis, we describe the potential of cell mechanical phenotyping for immune cell characterization, diagnosis, drug testing, as well as treatment of disease.

Decision support tool for cell therapy commercialization and business development

Leiden has a long history in the treatment of blood cell cancer. Research to find better therapies never stands still. One of the potential treatments currently being worked on is a ‘living medicine’.

Mechanical interactions between cells and their environment play an important role in many biological processes.

Promotor: Prof.dr. J. Kuiper, Co-Promotor: S.C.A. de Jager

The bacterial cell wall is a nearly universal structure that offers protection and gives the cell its shape. However, environmental stressors, such as cell wall-targeting antibiotics and hyperosmotic conditions, can induce bacteria to shift to a wall-deficient state. It is unknown whether the lack of…

BioSolar Cells is a five-year research project in which ten knowledge institutions and 45 private industries work together.

What distinguishes a tumour cell from a healthy cell? Researchers are trying to answer this question as precisely as possible. Certain differences could eventually lead to new therapies.

In this project innovative technology for photovoltaics cells is developed and analysis of ecological and health related risks are explicitly accounted for.

Cardiovascular disease is a major global burden and atherosclerosis is the main underlying pathological process.

The CMCB brings together microbial cell biology expertise in Leiden. It aims at optimal resonance between the groups and maximal exploitation of the available infrastructure and to develop innovative cross-boundary technology platforms.

The research within the Division of Cell Systems and Drug Safety, headed by Prof. Bob van de Water, is focused on novel therapeutic modalities and novel concepts in early drug discovery, in order to develop more effective and safer therapeutic strategies. We generate advanced cell and computational…

Promotor: R.C.M. Pelger Co-Promotor: G. van der Pluijm

Almost all bacteria are enveloped by a cell wall that provides cellular protection.

Acute cardiovascular syndromes, including myocardial infarction or stroke, are the principal cause of death in the Western society. The main underlying pathology of cardiovascular diseases is atherosclerosis, which is caused by the accumulation of lipids and inflammatory cells in the vessel wall, in…

Which brain cell does what, when Parkinson's disease arises? It won't be long before this jigsaw is solved piece by piece. Ahmed Mahfouz, computational biologist, combines bio-knowledge from Leiden with algorithms from Delft and is getting closer to finding the key.

This report concerns itself with terrorist technical innovation, particularly with regards to terrorists’ incorporation of emerging technologies into their practices. More specifically, it investigates, through the elaboration of a theoretical learning framework, how terrorist groups can adopt the practices…

The loss of T-cell homeostasis and tolerance towards self-antigens is the underlying cause of autoimmune and inflammatory diseases like atherosclerosis, rheumatoid arthritis or type I diabetes. Traditionally, the treatment of these diseases consisted on systemic immune suppression, which can entail…

This work describes the use of click-to-release chemistry to get spatiotemporal control over immunocytokine activity. Until now, immunocytokines (cytokines coupled to a tumor-targeting-moiety) remained active throughout the body, being able to bind their respective receptors, causing mild to severe…

Atherosclerosis, in particular rupture of atherosclerotic plaques, is the underlying cause of most heart attacks or strokes and is the leading cause of death worldwide.

Atherosclerosis is the most important underlying process that drives cardiovascular disease, and is characterized by an accumulation of cholesterol which triggers an inflammatory response in the vessel wall.

Atherosclerosis is the main underlying pathology of cardiovascular disease. Atherosclerosis is caused by an immune response which is directed against (modified) lipoproteins which accumulate in the vessel wall.

The word ¨pyroptosis¨ can be understood as ¨fiery falling¨, which describes the bursting of pro-inflammatory signals from the dying cell. Our observations indicate that pyroptosis also ¨spreads like wildfire¨ and once a cell dies via pyroptotic cell death, neighbouring cells are more prone to die as…

Cardiovascular syndromes are the major cause of death in Western societies.

Source: Biosens Bioelectron, Volume 74, pp. 233-42 (2015)

Cell adhesion signaling

We aim to engineer streptomycetes to fully exploit their potential for natural product productions, by a rational design and evolution approach.

From molecule, to cell, to organism. The high-level drug research at the Cell Observatory aimes at reducing the spread of cancer and developing new medicines for tuberculosis.

Epithelial-mesenchymal plasticity (EMP) and tumor cell migration play an important role in cancer progression, and an improved understanding of the mechanisms underlying these concepts is essential for developing new targeted approaches.

Treatment with special stem cells seems to be a good option for suppressing the immune system in organ transplants. Researchers are trying to learn from processes that take place in the human body during pregnancy.

A new therapy for the serious Graft-versus-Host disease

Visualization of biomolecules in living cells by tagging with gold nanoparticles.

In this research project, we aimed to obtain more insight in the role of mast cells in the immune driven disorders rheumatoid arthritis and atherosclerosis.

Promotores: Prof.dr. M.K. Richardson, Prof.dr. H.P. Spaink

There is a need for alternative methods to replace, reduce and refine (3R) animal experimentation. Combining experimental data from high-throughput in vitro studies with in silico modeling is a promising approach to unravel the effect of chemicals on living cells and to gain a better understanding of…

What is the role of cell wall-deficiency in the persistence of tuberculosis?

The first steps are made in personalised cancer vaccination strategies, which aim to induce a more specific immune response with fewer side effects. The activation of cytotoxic T cells is crucial for an effective immune response.

A new type of tripodal squaramide-based supramolecular hydrogels is developed and studied.

The emergence of complex diseases resulting from abnormal cell-cell signaling and the spread of infectious diseases caused by pathogens are significant threats to humanity. Unraveling the dynamic mechanisms underlying cell-cell signaling and infectious disease spreading is crucial for effective disease…

During embryonic growth, cells proliferate, differentiate, and collectively migrate to form different tissues at the right position and time in the body.

This functional genomics project aims at understanding the biology of the underlying mycelium differentiation and autolysis processes in much more detail.

Bacteria have the ability to alter their morphology in order to adapt to changing environments.

Promotor: Huub J.M. de Groot, Co-promotor: Francesco Buda