941 search results for “protein” in the Public website
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Covalent inhibitors of G protein-coupled receptors: the case of adenosine receptors
Supervisor: Xue Yang
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environmental cues: the adaptive qualities of chromatin compaction proteins
Promotor: M. Ubbink, Co-Promotor: R. T. Dame
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Innovative analytical approaches for protein aggregates and other particulate matter impurities
Developing robust and comprehensive analytical methods to size and quantify subvisible particles in biopharmaceutical products.
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Quantifying nucleosome dynamicsand protein binding with PIE-FCCS and spFRET
In this thesis I describe the results of Pulsed Interleaved Excitation and Fluorescence (Cross) Correlation Spectroscopy (PIE-F(C)CS) combined with single-pair Förster Resonance Energy Transfer (spFRET) used to study dynamics in single nucleosomes, which depends on subtle differences in the length of…
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Relative quantification of proteasome activity by activity-based protein profiling and LC-MS/MS
Activity-based protein profiling (ABPP) is a functional proteomics technique for directly monitoring the expression of active enzymes in cell extracts and living cells. The technique relies on irreversible inhibitors equipped with reactive groups (warheads) that covalently attach to the active site…
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Indanes-Properties, Preparation, and Presence in Ligands for G Protein Coupled Receptors
The indane (2,3-dihydro-1H-indene) ring system is an attractive scaffold for biologically active compounds due to the combination of aromatic and aliphatic properties fused together in one rigid system.
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Protein arginine methyltransferases as modulators of lipid metabolism and inflammation and the relevance for atherosclerosis
Acute cardiovascular clinical events such as myocardial infarction and cerebral stroke represent the major cause of death in Western societies. These pathologies are primarily resulting from atherosclerosis, a progressive condition characterized by the accumulation of lipids, immune cells, and fibrous…
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Artificial metallo-proteins for photocatalytic water splitting: stability and activity in artificial photosynthesis
Climate change is one of the largest challenges faced by humanity. To combat this research into alternatives to fossil fuels is ongoing. Dihydrogen is considered a good alternative fuel, since its burning only forms water.
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The use of activity based protein profiling to study proteasome biology
Promotor: H.S. Overkleeft, Co-Promotor: B.I. Florea
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Measurement of the average mass of proteins adsorbed to a nanoparticle by using a suspended microchannel resonator
SMR can be used to measure the mass of adsorbed protein to nanoparticles with a high precision in the presence of free protein.
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The activation mechanisms of G protein-coupled receptors: the case of the adenosine A2B and HCA2/3 receptors
Promotor: A.P. IJzerman
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Lipid to protein ratio plays an important role in the skin barrier function of atopic eczema patients
The barrier function of the skin is primarily provided by the stratum corneum (SC), the outermost layer of the skin. Skin barrier impairment is thought to be a primary factor in the pathogenesis of atopic eczema (AE).
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Structural and functional analysis of proteins involved in natural product biosynthesis and morphological differentiation in Streptomyces
Streptomyces present a valuable platform for natural product discovery. Lugdunomycin is a novel angucycline-derived polyketide from Streptomyces sp QL37, with unprecedented skeleton and antimicrobial activity.
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Allosteric modulation by sodium ions and amilorides of G protein-coupled receptors
Promotor: A.P. IJzerman
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Allosteric modulation by sodium ions and amilorides of G protein- coupled receptors
Promotor: A.P. IJzerman
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Protein-polyelectrolyte interactions: Monitoring particle formation and growth by nanoparticle tracking analysis and flow imaging microscopy
The purpose of this study was to investigate the formation and growth kinetics of complexes between proteins and oppositely charged polyelectrolytes.
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Sugars and their Interference with Light Scattering-Based Analysis of Protein Formulations
PURPOSE: In the present study we investigated the root-cause of an interference signal (100-200 nm) of sugar-containing solutions in dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) and its consequences for the analysis of particles in biopharmaceutical drug products.
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and crystallographic studies of the Sso10a2 and human C1 inhibitor protein
Promotor: J.P. Abrahams, Co-Promotor: N.S. Pannu
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space smarter: Artificial intelligence in drug design for G protein-coupled receptors
Over several decades, a variety of computational methods for drug discovery have been proposed and applied in practice. With the accumulation of data and the development of machine learning methods, computational drug design methods have gradually shifted to a new paradigm, i.e. deep learning methods…
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Lipid to protein ratio plays an important role in the skin barrier function of atopic eczema patients
OBJECTIVES: To determine whether the lipid/protein ratio and the total dry SC mass per surface area are related with the skin barrier function of controls and AE patients.
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between the adenosine A2B receptor and the C-terminus of different G protein α-subunits.
Source: Purinergic Signal., Volume 10, Issue 3, pp. 441-53 (2014)
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Wrestling with SUMO proteins
The work done by SUMO proteins in the cells of our body is of vital importance. Molecular cell biologist Alfred Vertegaal has been studying these proteins for nine years, first with a Veni subsidy and then with a prestigious Vidi subsidy.
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Research shows protein movement is important
Researchers led by Professor of Chemistry Marcellus Ubbink have recently published a study in Proceedings of the National Academy of Sciences (PNAS) about the dynamics of an important redox enzyme. This work was accomplished thanks to an NWO VICI subsidy granted to Professor Ubbink.
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New force measured between proteins
Proteins organize themselves around our body cells through a self-induced force. They indent the cell membrane, which makes them roll towards each other. This discovery provides new insights into processes like nutritional uptake and brain signaling, as well as into such diseases as Alzheimer’s. Publication…
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A cobalt cage for proteins
A new paramagnetic probe has been synthesized and applied to proteins.
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Essential protein for metastasis identified
An essential protein that regulates our heart beat turns out to be important for cancer cells as well. The discovery might open novel treatment strategies for fighting metastasis. Publication in Science Signaling on April 4th.
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NPxxY(x)(5,6)F motif and helix 8 of the adenosine A(2B) receptor in G protein coupling
Source: Biochem Pharmacol, Volume 95, Issue 4, pp. 290-300 (2015)
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Shaping proteins to understand chaperone-related diseases
Alireza Mashaghi and his research team have created a new framework to understand shapes of proteins and DNA. With this framework, many diseases can be understood better.
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Halting protein degradation may contribute to new cancer treatment
Chemist Gerjan de Bruin has designed a method of slowing down the degradation process of proteins in cells. This may contribute to new cancer medicines with fewer side-effects. PhD defence on 1 June.
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Versatile antiviral proteins discovered with supercomputer
A single tiny molecule that can destroy flu, corona, HIV and Zika viruses? Yes, it really does exist. Biophysicist Niek van Hilten, who will receive his doctorate on 14 September, contributed to this discovery.
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How an Alzheimer related protein forms plaques
Neurological diseases, such as Alzheimer's disease, are characterised by aggregates of protein in the brain. The connection of these aggregates to the disease itself is unclear. Martina Huber, Enrico Zurlo and colleagues published a new method to monitor the formation of these aggregates.
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New method to catch notorious Alzheimer protein
A new biophysical technique enables scientists to study the behavior of Aβ-peptide. This protein is known to play a role in Alzheimer’s, but for effective medication we need to know exactly what it does. Publication in Journal of Biological Physics on March 16th.
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Old protein distinguishes bone fragments of Neanderthals
Bone remains that are thousands of years old are often too fragmented to be identified. PhD candidate Frido Welker is the first person to be able to distinguish human bones from one another on the basis of old proteins. PhD defence 18 May.
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Spring Symposium: from proteins to data
During the annual LACDR Spring Symposium, the audience chose PhD student Rob van Wijk as best speaker. Furthermore, six PhD students won a poster prize. Matthias P. Mayer of Heidelberg University opened the symposium with a keynote lecture about chaperone proteins.
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Biological Origami at molecular level: folding a single protein
Human cells are protecting their proteins from unfolding and aggregating. That's what biophysicist Alireza Mashaghi and his team discovered after seven years of in-depth research into the folding mechanisms of proteins. With an unprecedented approach, the team was able to study the folding of a single…
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Proteins are not distributed equally in ancient teeth
In a study led by Leiden alumnus Jan Dekker, based on his research master’s thesis, he applied Mass Spectrometry Imaging to archaeological human teeth. The research shows that there are large differences in the intensity of proteins across the teeth, opening new avenues of investigation.
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Essentiality of conserved amino acid residues in β-lactamase
Evolution acts via mutations in amino acid sequences. Substitution of essential amino acids leads to a nonfunctional protein. l.
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Inhibiting protein production potentially a weapon against Alzheimer’s
Inhibiting specific protein production in the human body can serve as a crucial weapon in the fight against Alzheimer's, Eline van Maanen believes. PhD defence 23 November.
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Parkinson Protein α-Synuclein Binds Surprisingly Strong with Membrane
Α-synuclein, a protein associated with Parkinson’s disease, proves to bind with membranes in a surprisingly efficient way. It confirms scientists’ suspicion of the protein’s leading role in the transmission of neurotransmitters between nerve cells in the brain. Publication in PLoS ONE.
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Leiden iGEM students win prize with squid protein
The Leiden students who participated in the iGEM synthetic biology competition have won the prize for best production. They developed a way to have bacteria produce the substance suckerine, a protein derived from the Humboldt squid. By doing so, they want to help heal burn wounds. ‘The announcement…
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Louise Jawerth gets vidi for understanding pesky protein fibers
Mysterious protein fibers show up in the brain cells of people suffering from Alzheimer’s' disease and other neurodegenerative disorders. Leiden physicist Louise Jawerth has been awarded a Vidi grant to find out how these fibers form in the first place.
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Student team hopes to heal skin with squid protein
Eleven ambitious Leiden students hope to heal burns with a special substance from squid teeth. They are taking part in the international iGEM competition. They need to raise almost 10,000 euros to fund their project this summer.
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Click-to-release for immune cell activation
This work describes the use of click-to-release chemistry to get spatiotemporal control over immunocytokine activity. Until now, immunocytokines (cytokines coupled to a tumor-targeting-moiety) remained active throughout the body, being able to bind their respective receptors, causing mild to severe…
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The structure of the cytochrome P450cam-putidaredoxin complex determined by paramagnetic NMR spectroscopy and crystallography
Promotor: Prof.dr. M. Ubbink
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Lars Jeuken
Science
l.j.c.jeuken@lic.leidenuniv.nl | +31 71 527 4755
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How cells determine the fate of proteins (and can we do it too?)
Cells in our bodies are often threatened by errors in our own proteins. The FLOW consortium, comprising scientists from various institutions including Leiden, is poised to meticulously map out for the first time how cells control proteins, correcting or removing faulty ones. This endeavour holds promise…
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Analysis of sub-visible particles in complex injectable formulations
Promotors: W. Jiskoot; G.J. Witkamp, Co-promotor: M.R. Nejadnik
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Reading through proteins with graphene: NWO Vidi grant awarded to Dr. Grégory Schneider
While there are numerous and extremely advanced methods to sequence the genome, only a few methods exist to sequence the proteome. The Vidi project of Grégory Schneider promises to shed light on the most difficult paradigm of proteomics: achieving an error-free determination of the sequence of single…
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Proteins and DNA loops: Science-Groot grant for Remus Dame
How do our cells keep DNA stored away? To find out, biochemist Remus Dame received a 3 million euro Science-Groot grant. Leiden University will share the grant with TU Delft, VU Amsterdam and the Hubrecht Institute.
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Experimental drug BIA 10-2474 deactivates proteins in human nerve cells
At high doses, drug candidate BIA 10-2474 binds not only to the protein that it targets, but to other proteins as well. It thus deactivates proteins that are involved in the metabolism of nerve cells. This is what an international group of researchers from Leiden University and Erasmus MC, among others,…