The Microscopy Unit houses, maintains and coordinates most of the microscopy equipment of the IBL. The available equipment ranges from conventional light and fluorescence microscopes, to confocal laser scanning and electron microscopes. In addition, infrastructure is available for histology, including…

Promotor: T. Schmidt

Single-Molecule Microscopy (SMM) techniques constitute a group of powerful imaging tools that enable researchers to study the dynamic behavior of individual molecules.

This is a yearly event where we move in one week from the physics of light through the composition of the microscope, fluorescence microscopy and end up with some image analysis All the lectures are backed by practicals to really put your obtained knowledge into practice

This thesis presents insights from our study of various correlated electron systems with a scanning tunneling microscope (STM). In ordinary metals, electron-electron interactions exist, but get substantially screened due to the sheer number of electrons.

Electron microscopy has become an extremely important techniquein a wide variety of elds.

The aim of this facility is to accelerate biomedical research by providing access to advanced light and electron microscopy and by sharing expertise about sample preparation, imaging modalities, data storage and image analysis.

This thesis described the development of novel scanning tunneling microscopy techniques to investigate strongly correlated electronic states in quantum matter.

In surface science there is great effort to move from studying simple, flat model surfaces in vacuum to investigating more complex model catalysts in gas environments (in situ). This thesis gives three examples of such studies using microscopy and spectroscopy.

Promotor: Prof.dr. J.M. van Ruitenbeek, Prof.dr. R.M. Tromp

In this thesis, we describe the latest advances in SQUID-detected Magnetic Resonance Force Microscopy (MRFM).

In this thesis, we show how MRFM can usefully contribute to the field of condensed-matter.

In this thesis we investigated the ability of two-photon multifocal microscopy for single-molecule microscopy in live cells and organisms.

Intrigued by the way cells autonomously regulate their fate, we strive to understand and visualize cellular processes as the basis of Cell Signaling.

In this thesis we use Josephson and noise scanning tunneling microscopy for the study of conventional, unconventional (iron-based) and disordered superconductors. On the one hand, Josephson scanning tunneling microscopy allows us to directly visualize the superfluid density with high spatial resolut…

The dissertation describes TEM experiments on heterogeneous catalysts.

Promotores: Prof.dr. H.P. Spaink & Prof.dr. P.C.W. Hogendoorn, Co-promotor: Dr. M.J.M. Schaaf

Promotor: T.H. Oosterkamp

Promotores: Prof.dr. C.U. Keller, Prof.dr. H.C. Gerritsen

n this PhD thesis, the recombination of different atomic lattices in stacked 2D materials such as twisted bilayer graphene is studied. Using the different possibilities of Low-Energy Electron Microscopy (LEEM), the domain forming between the two atomic layers with small differences is studied.

We explore the possibilities to combine magnetic resonance techniques with atomic force microscopy together in a single microscope: the MRI-AFM, also called Magnetic Resonance Force Microscopy (MRFM).

In this Ph.D. thesis we study the interaction of low energy electrons with thin materials, namely layered materials (graphene, hexagonal boron nitride, molybdenum disulfide) and organic films. At these low energies the quantum mechanical wavelength of the electron wave function is in the order of a…

High throughput microscopy

Establishing human induced pluripotent stem cell (iPSC) CRISPR-Cas9-based fluorescent reporter cell lines for NF-kB signalling. We will use this to enable studying the dynamic activation and downstream signalling of Toll-like receptor and cytokine signalling in iPSC-derived differentiated cells in a…

The purpose of this study was to investigate the formation and growth kinetics of complexes between proteins and oppositely charged polyelectrolytes.

Promotor: B. van de Water

Promotor: B. van de Water

The Netherlands Organisation for Scientific Research (NWO) has made a subsidy of 17 million euros available to further develop a Netherlands network for electron microscopy (NEMI). The network comprises five UMCs and eight universities, with Utrecht in the coordinating role. From Leiden, the Institute…

Chinese and Leiden scientists came together in Leiden to study the intricacies of modern visual techniques.

In this thesis the combinatorial use of bioorthogonal labelling and Electron Microscopy (EM)-based imaging techniques is explored to enable observations of specific molecular targets in their ultrastructural context within the cell.

Advanced microscopy to understand life and fight disease: that’s the goal of the new NL-BioImaging network that will develop and integrate state-of-the-art microscopy technologies and services. Researchers from all Dutch universities, including Leiden University and the Leiden University Medical Centre,…

From surface science towards industrial practice using high-pressure scanning tunneling microscopy.

Molecular materials have been a subject of interest in fundamental research and applications for decades, and have been studied as bulk crystals, (thin) films and as individual molecules, due to the large variety in their properties. This dissertation explores pentacene crystals near the two-dimensional…

Due to the corona crisis, they had to switch to online education halfway during their course: associate professor Marcel Schaaf and PhD candidate Michiel Hooykaas of the Institute of Biology Leiden talk about digital practicals, online lectures and their biggest obstacle: exams.

Promotor: Prof.dr. J.P. Abrahams, Prof.dr. M. van Heel

‘Microscopy is by far the least understood, most inefficiently operated, and the most abused of all laboratory instruments,’ reads the quote on the office wall of microscopy unit supporters Joost Willemse en Gerda Lamers. It describes exactly why the two developed the microscopy course for starting…

On February 2nd the book Beelden zonder weerga appears, written by professor in science history Dirk van Delft and biochemist Ton van Helvoort. They describe the rich history of electron microscopy, which comes to a conclusion in the final chapter with the current state-of-the-art ESCHER microscope…

Materials with strongly correlated electrons show some of the most mysterious and exotic phases of quantum matter, such as unconventional superconductivity, quantum criticality and strange metal phase.

In this thesis, the focus lies on studying glucocorticoid receptor dynamics in living cells with the aim of understanding how this transcription factor finds its DNA target sites to regulate transcription.

Bacteria are everywhere. They are the most abundant organisms on earth and impact all aspects of our lives. They determine our health and shape our environment. Ariane Briegel, professor of Ultrastructural Biology, freezes bacteria super fast to gain a true-to-nature image of the internal and external…

On 3 and 4 June 2015 a delegation from the biotechnology and biomedicine center in Prague (BIOCEV) visited the Faculty of Science in Leiden, as well as the Netherlands Centre for Electron Microscopy (NeCEN). The delegation was interested to see the potential for collaboration in the field of cryo transmission…

Moving an electron microscope of 2000 kg is a delicate challenge. The highly sensitive instrument needed to be moved to a new measurement hall, but even a tiny bump could damage it. After a few nerve-racking weeks of preparing the move and reinstalment, the researchers finally have a verdict: the instrument…

The High Troughput Microscopy Dutch Flagship Node that includes the Leiden Cell Observatory Screening platform is now officially ratified as a Euro-BioImaging (EuBI) Node Candidate

Bacteria have the ability to alter their morphology in order to adapt to changing environments.